Composite

Part:BBa_K2680274

Designed by: Ethan M Jones   Group: iGEM18_William_and_Mary   (2018-10-06)


3G deGFP-ssra

3G deGFP ssrA is flanked by prefix sticky end C and suffix sticky end D in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].

deGFP is a destabalized form of GFP with a ssrA degradation tag. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 41
    Illegal BsaI site found at 95
    Illegal BsaI.rc site found at 819
    Illegal BsaI.rc site found at 875


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